4RNG

Crystal structure of a bacterial homologue of SWEET transporters

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-18
Detector	RAYONIX MX-225
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	52.880, 96.155, 119.797
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.400
Rwork	0.251
R-free	0.29360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qnc
RMSD bond length	0.010
RMSD bond angle	1.312
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	23655
Completeness [%]	94.5	95.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.2	293	2% 3/4 EO/OH (Pentaerythritol ethoxylate), 0.1M Lithium sulphate, 0.1M Sodium citrate pH 5.2, Lipidic cubic phase, temperature 293K