4RMS
Crystal structure of the D53N Beta-2 Microglobulin mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 55.020, 28.900, 67.155 |
| Unit cell angles | 90.00, 101.93, 90.00 |
Refinement procedure
| Resolution | 26.454 - 1.700 |
| R-factor | 0.1968 |
| Rwork | 0.191 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yxf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.133 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.454 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.096 | 0.336 |
| Number of reflections | 10917 | |
| <I/σ(I)> | 5.4 | 2.1 |
| Completeness [%] | 94.5 | 92.1 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 27% PEG4000, 15% glycerol, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
