4RMQ
Crystal structure of the D59N Beta-2 Microglobulin mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 54.770, 28.930, 67.256 |
| Unit cell angles | 90.00, 101.76, 90.00 |
Refinement procedure
| Resolution | 23.236 - 1.461 |
| R-factor | 0.1689 |
| Rwork | 0.166 |
| R-free | 0.19270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yxf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.198 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.240 | 1.490 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Rmerge | 0.048 | 0.202 |
| Number of reflections | 17472 | |
| <I/σ(I)> | 10.5 | 3.6 |
| Completeness [%] | 96.3 | 96 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 27% PEG4000, 15% glycerol, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
