4RMK
Crystal structure of the Olfactomedin domain of latrophilin 3 in P65 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 65 |
| Unit cell lengths | 85.066, 85.066, 66.083 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.835 - 1.606 |
| R-factor | 0.1665 |
| Rwork | 0.165 |
| R-free | 0.19040 |
| Structure solution method | Native-SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.127 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.650 |
| High resolution limit [Å] | 1.606 | 1.610 |
| Number of reflections | 35190 | |
| Completeness [%] | 99.1 | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbach under-oil | 9 | 293 | 15-25 % PEG 8000, 50-300 mM MgCl2, 100 mM TAPS pH 9.0, Microbach under-oil, temperature 293K |
