4RMB
Crystal structure of keratin 4 binding domain of surface adhesin Srr-1 of S.agalactiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-27 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97927 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.050, 71.890, 47.320 |
| Unit cell angles | 90.00, 96.03, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| Rwork | 0.222 |
| R-free | 0.24290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mbo |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.950 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.066 | 0.660 |
| Number of reflections | 27350 | |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 95.4 | 80.7 |
| Redundancy | 5.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293.15 | 25% (w/v) PEG 3350 (Sigma), 100 mM Lithium chloride and 100 mM Imidazole, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
