4RM6
Crystal structure of Hemopexin Binding Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 93.400, 177.130, 54.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.390 - 1.600 |
| R-factor | 0.1892 |
| Rwork | 0.188 |
| R-free | 0.21060 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.680 | 1.640 |
| High resolution limit [Å] | 1.550 | 2.320 | 1.550 |
| Number of reflections | 131352 | ||
| <I/σ(I)> | 18.58 | 1.46 | |
| Completeness [%] | 98.6 | 99 | 96.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | Reservoir solution: 0.2 M MgCl2, 0.1 M HEPES pH 7.5, 30% w/v PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
