4RLZ
Crystal structure of Norovirus OIF P domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.495, 112.663, 59.711 |
| Unit cell angles | 90.00, 108.50, 90.00 |
Refinement procedure
| Resolution | 31.551 - 1.190 |
| R-factor | 0.1412 |
| Rwork | 0.140 |
| R-free | 0.15950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pun |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.131 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.210 |
| High resolution limit [Å] | 1.190 | 1.190 |
| Rmerge | 0.056 | 0.316 |
| Number of reflections | 197918 | |
| <I/σ(I)> | 25.24 | 4.1 |
| Completeness [%] | 94.9 | 86.9 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 6.5 | 290 | 0.1M Mes, pH6.5, 0.25M Ammonium Sulfate, 18% PEG 3350, LIQUID DIFFUSION, temperature 290K |
