4RLN

Hen egg-white lysozyme solved from serial crystallography at a synchrotron source, data processed with nXDS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	293
Detector technology	PIXEL
Collection date	2014-04-13
Detector	PSI PILATUS 6M
Wavelength(s)	1.32
Spacegroup name	P 43 21 2
Unit cell lengths	79.000, 79.000, 38.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.980 - 2.170
R-factor	0.19526
Rwork	0.193
R-free	0.23846
Structure solution method	MIRAS
RMSD bond length	0.007
RMSD bond angle	1.135
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	SHARP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	18.000
High resolution limit [Å]	2.170
Number of reflections	6399
Completeness [%]	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	293	20% (w/v) NaCl, 6% (w/v) PEG6000, 0.5 M NaCl, pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K