4RLM

Hen egg-white lysozyme solved from serial crystallography at a synchrotron source, data processed with CrystFEL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	293
Detector technology	PIXEL
Collection date	2014-04-13
Detector	PSI PILATUS 6M
Wavelength(s)	1.32
Spacegroup name	P 43 21 2
Unit cell lengths	78.600, 78.600, 37.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.580 - 1.900
R-factor	0.19775
Rwork	0.196
R-free	0.24276
Structure solution method	MIRAS
RMSD bond length	0.008
RMSD bond angle	1.169
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	SHARP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	55.580
High resolution limit [Å]	1.900
Number of reflections	9320
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	293	20% (w/v) NaCl, 6% (w/v) PEG6000, 0.5 M NaCl, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K