4RLA
ALTERING THE BINUCLEAR MANGANESE CLUSTER OF ARGINASE DIMINISHES THERMOSTABILITY AND CATALYTIC FUNCTION
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-01 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 32 |
| Unit cell lengths | 89.000, 89.000, 115.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.900 * |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | RAT LIVER ARGINASE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.800 * |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.010 |
| High resolution limit [Å] | 2.900 | 2.940 |
| Rmerge | 0.094 | 0.500 |
| Total number of observations | 24335 * | |
| Number of reflections | 18429 | |
| <I/σ(I)> | 4.5 | 1.1 |
| Completeness [%] | 81.4 | 85.4 |
| Redundancy | 1.3 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 4 * | 12 - 18% PEG 8000, 50 MM BICINE PH = 8.5, 0.05% AZIDE, 1 MM MNCL2 SOAKED FOR 1 WEEK IN 15MM EDTA + DPA |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | |
| 2 | 1 | drop | bicine | 50 (mM) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | reservoir | PEG8000 | 14 (%(w/v)) | |
| 5 | 1 | reservoir | bicine | 50 (mM) | |
| 6 | 1 | reservoir | 1 (mM) | ||
| 7 | 1 | reservoir | sodium azide | 0.05 (%(w/v)) |
