4RL8

Crystal structure of the COG4313 outer membrane channel from Pseudomonas putida F1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	101.051, 120.309, 131.396
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.975 - 2.300
R-factor	0.2151
Rwork	0.213
R-free	0.25310
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.005
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	71517
Completeness [%]	100.0	96.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	295	50 mM sodium acetate, 0.2 M ammonium phosphate, 40% PEG400, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K