4RL5

Crystal structure of the Arabidopsis exocyst subunit exo70 family protein A1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Detector technology	CCD
Collection date	2014-05-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	P 21 21 21
Unit cell lengths	55.108, 72.117, 327.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.521 - 3.100
R-factor	0.3258
Rwork	0.324
R-free	0.34790
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.818
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000
Number of reflections	27255
Completeness [%]	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	200 mM di-ammonium tartrate, pH7.0, and 21.5% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K