4RKR
Crystal structure of LacI family transcriptional regulator from Arthrobacter sp. FB24, target EFI-560007, complex with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-10 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.936, 124.912, 117.043 |
| Unit cell angles | 90.00, 96.51, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.17387 |
| Rwork | 0.171 |
| R-free | 0.25183 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rkq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.230 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.141 | |
| Number of reflections | 52910 | |
| <I/σ(I)> | 7.5 | 1.4 |
| Completeness [%] | 99.1 | 98.6 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | Protein: 10 mM bis-tris, 500 mM NaCl, 10% glycerol, 5 mM DTT reservoir: 0.1 M magnesium chloride, 0.1 M MES:HAOH, pH 6.5, 30% (w/v) PEG 400; cryoprotection: reservoir plus 20 mM lactose; vapor diffusion, sitting drop, temperature 294K |
