4RKQ
Crystal structure of LacI family transcriptional regulator from Arthrobacter sp. FB24, target EFI-560007
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-26 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.244, 125.126, 116.422 |
Unit cell angles | 90.00, 96.68, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.903 |
R-factor | 0.15591 |
Rwork | 0.154 |
R-free | 0.20704 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.012 |
RMSD bond angle | 1.540 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELX |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.112 | 0.630 |
Number of reflections | 82386 | |
<I/σ(I)> | 14.5 | 2.4 |
Completeness [%] | 100.0 | 95.7 |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | protein: 10 mM bis-tris, 500 mM NaCl, 10% glycerol, 5 mM DTT reservoir: 0.1 M magnesium chloride, 0.1 M MES:NaOH, pH 6.5, 30% (w/v) PEG 400; cryoprotection: reservoir; vapor diffusion, sitting drop, temperature 294K |