4RJX
Crystal structure of the OprO mutant protein F62Y/D114Y
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-10 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.92 |
| Spacegroup name | H 3 |
| Unit cell lengths | 87.479, 87.479, 159.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.300 - 1.540 |
| R-factor | 0.1555 |
| Rwork | 0.154 |
| R-free | 0.17860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.300 | 1.580 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Number of reflections | 67047 | |
| Completeness [%] | 99.6 | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 50 mM sodium acetate, 0.225 M ammonium sulphate, 12% PEG4000, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
