4RJW
Crystal structure of Pseudomonas aeruginosa OprO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-01 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 86.971, 86.971, 158.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.405 - 1.520 |
| R-factor | 0.1511 |
| Rwork | 0.150 |
| R-free | 0.16770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.156 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Number of reflections | 68154 | |
| Completeness [%] | 99.1 | 81.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 50 mM sodium acetate, 0.2 M ammonium sulphate, 10-15% PEG4000, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
