4RJE
Aerococcus viridans L-lactate oxidase mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.593, 124.355, 106.885 |
| Unit cell angles | 90.00, 124.29, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.650 |
| R-factor | 0.15336 |
| Rwork | 0.152 |
| R-free | 0.17956 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.538 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.371 | |
| Number of reflections | 155981 | |
| <I/σ(I)> | 26.4 | 4.1 |
| Completeness [%] | 98.2 | 95.1 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | Drop contained 1 ul 10 mg/ml LOX in 50 mM potassium phosphate pH 7.0 and 1 ul well. Well contained 50 mM pyruvate, 50 mM Tris (pH 8.0) and 30% PEG, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
