4RIM
Native structure of intercalation-locked DNA tetraplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-30 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 25.310, 25.310, 167.832 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| R-factor | 0.30146 |
| Rwork | 0.299 |
| R-free | 0.30173 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.216 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0085) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.940 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.076 | 0.076 |
| Number of reflections | 1595 | |
| <I/σ(I)> | 2.2 | 1.4 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 7.7 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 300 | 100 mM BaCl2, 20% MPD, 30 mM NaCaco pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
