4RIG
Chimeric Glycosyltransferase LanGT2S8Ac
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.943, 59.109, 63.111 |
| Unit cell angles | 79.30, 71.72, 87.69 |
Refinement procedure
| Resolution | 46.010 - 1.900 |
| R-factor | 0.20497 |
| Rwork | 0.203 |
| R-free | 0.24055 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | LanGT2 wild type |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.187 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.010 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.034 |
| Number of reflections | 52458 |
| <I/σ(I)> | 14.2 |
| Completeness [%] | 93.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 278 | 34% PEG 3350 0.17 M MgCl2 16 mM L-proline 0.1 M HEPES/NaOH, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
