4RIE
Landomycin Glycosyltransferase LanGT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 61 |
| Unit cell lengths | 75.992, 75.992, 214.314 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.810 - 2.162 |
| R-factor | 0.19975 |
| Rwork | 0.198 |
| R-free | 0.24198 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p6p |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.334 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 214.310 | 2.280 |
| High resolution limit [Å] | 2.160 | 2.160 |
| Rmerge | 0.043 | 0.490 |
| Number of reflections | 37364 | |
| <I/σ(I)> | 40.4 | 5.3 |
| Completeness [%] | 100.0 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 1.3 M sodium citrate 0.1 M HEPES/NaOH, pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
