4RI6
Crystal structure of poplar glutathione transferase F1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-22 |
| Detector | MAR555 FLAT PANEL |
| Wavelength(s) | 0.8150 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.838, 65.936, 109.842 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.281 - 1.523 |
| R-factor | 0.1528 |
| Rwork | 0.151 |
| R-free | 0.18270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gnw |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.262 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.281 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.034 | 0.200 |
| Number of reflections | 65579 | |
| <I/σ(I)> | 40.6 | 8.4 |
| Completeness [%] | 99.2 | 95.2 |
| Redundancy | 11 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH UNDER OIL | 6.5 | 277 | 30% w/v PEG4000, 100 mM MES sodium, pH 6.5, MICROBATCH UNDER OIL, temperature 277K |
