4RI6
Crystal structure of poplar glutathione transferase F1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-08-22 |
Detector | MAR555 FLAT PANEL |
Wavelength(s) | 0.8150 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.838, 65.936, 109.842 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.281 - 1.523 |
R-factor | 0.1528 |
Rwork | 0.151 |
R-free | 0.18270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gnw |
RMSD bond length | 0.009 |
RMSD bond angle | 1.262 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.281 | 1.550 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.034 | 0.200 |
Number of reflections | 65579 | |
<I/σ(I)> | 40.6 | 8.4 |
Completeness [%] | 99.2 | 95.2 |
Redundancy | 11 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH UNDER OIL | 6.5 | 277 | 30% w/v PEG4000, 100 mM MES sodium, pH 6.5, MICROBATCH UNDER OIL, temperature 277K |