4RHS

Crystal structure of GD2 bound PltB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-23
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	68.257, 96.751, 104.002
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.046 - 1.921
R-factor	0.1763
Rwork	0.175
R-free	0.20540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.162
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: dev_1701))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.050	1.990
High resolution limit [Å]	1.920	1.920
Number of reflections	51223
Completeness [%]	95.6	93.04

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	297	26% (w/v) PEG1500 and 0.1 M sodium acetate, pH5.0 soaked with GD2, VAPOR DIFFUSION, HANGING DROP, temperature 297K