4RHK

Crystal structure of T. brucei arginase-like protein in an oxidized form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	H 3 2
Unit cell lengths	153.148, 153.148, 85.426
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.657 - 2.380
R-factor	0.2282
Rwork	0.227
R-free	0.25600
Structure solution method	PDB entry 4RHI
RMSD bond length	0.002
RMSD bond angle	0.622
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.470
High resolution limit [Å]	2.380	5.130	2.380
Rmerge	0.101
Number of reflections	15468
<I/σ(I)>	31.429
Completeness [%]	99.9	99.6	99.8
Redundancy	18.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	294	2% (v/v) tacsimate, pH 7.4, 5% (v/v) 2-propanol, 0.1 M imidazole, 8% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 294K