4RHI

Crystal structure of SeMet-labeled wild-type T. brucei arginase-like protein in P321 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9788
Spacegroup name	P 3 2 1
Unit cell lengths	139.009, 139.009, 90.509
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.360 - 2.550
R-factor	0.2228
Rwork	0.221
R-free	0.26060
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.628
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL2Map
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.550	5.490	2.550
Number of reflections	33070
Completeness [%]	100.0	99.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	294	0.1 M HEPES, pH 7.4, 10% PEG 3350, 5% 2-methyl-2,4-pentanediol, VAPOR DIFFUSION, SITTING DROP, temperature 294K