4RHF
Crystal structure of UbiX mutant V47S from Colwellia psychrerythraea 34H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97952 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 97.600, 97.600, 97.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 97.600 - 1.764 |
| R-factor | 0.23759 |
| Rwork | 0.237 |
| R-free | 0.25669 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.229 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MERLOT |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.600 | 1.860 |
| High resolution limit [Å] | 1.764 | 1.764 |
| Rmerge | 0.136 | 0.320 |
| Number of reflections | 30737 | |
| <I/σ(I)> | 26.8 | 13.7 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 40.5 | 39.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 0.1 M tri-sodium citrate, pH 5.4, 0.5 M ammonium sulfate, 1.2 M lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
