4RGZ

Crystal structure of recombinant prolidase from Thermococcus sibiricus at P21221 spacegroup

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 2 21
Unit cell lengths	97.600, 123.720, 136.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.480 - 2.600
R-factor	0.22438
Rwork	0.221
R-free	0.29593
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.877
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.600
Rmerge	0.077
Number of reflections	51209

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	COUNTER-DIFFUSION			Counter diffusion