4RGP

Crystal Structure of Uncharacterized CRISPR/Cas System-associated Protein Csm6 from Streptococcus mutans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-26
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.97927
Spacegroup name	P 21 21 21
Unit cell lengths	75.511, 78.238, 92.269
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.735 - 2.299
R-factor	0.2
Rwork	0.196
R-free	0.24300
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.613
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((phenix.refine: dev_1745))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	24863
<I/σ(I)>	11.5	2.1
Completeness [%]	99.6	99.2
Redundancy	5	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.2 M Calcium acetate, 0.1 M sodium cacodylate pH 6.5, 40 %(w/v) PEG300, VAPOR DIFFUSION, SITTING DROP, temperature 289K