4RGG

Structure of the lactococcal phage 1358 receptor binding protein in complex with GlcNAc-1P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-03
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 3
Unit cell lengths	165.910, 165.910, 165.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.890 - 2.150
R-factor	0.181
Rwork	0.180
R-free	0.20400
Structure solution method	MIR
Starting model (for MR)	4l92
RMSD bond length	0.020
RMSD bond angle	1.893
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	2.210
High resolution limit [Å]	2.150	2.150
Rmerge	0.089	0.810
Number of reflections	81936
<I/σ(I)>	20.5	2.43
Completeness [%]	99.9	99.9
Redundancy	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.2	293	20% w/v polyethyleneglycol 1000, 100 mM sodium/potassium phosphate buffer, pH 6.2, 200 mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K