4RGG
Structure of the lactococcal phage 1358 receptor binding protein in complex with GlcNAc-1P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 165.910, 165.910, 165.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.890 - 2.150 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.20400 |
| Structure solution method | MIR |
| Starting model (for MR) | 4l92 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.893 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.089 | 0.810 |
| Number of reflections | 81936 | |
| <I/σ(I)> | 20.5 | 2.43 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 293 | 20% w/v polyethyleneglycol 1000, 100 mM sodium/potassium phosphate buffer, pH 6.2, 200 mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
