4RGE

Crystal structure of the in-line aligned env22 twister ribozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Detector	PSI PILATUS 6M
Spacegroup name	P 61 2 2
Unit cell lengths	64.071, 64.071, 578.773
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	55.487 - 2.890
R-factor	0.1796
Rwork	0.177
R-free	0.22290
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.335
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: dev_1760))

Data quality characteristics

	Overall
Low resolution limit [Å]	55.500
High resolution limit [Å]	2.889
Number of reflections	17191
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.9	293	0.1 M imidole, pH 7.9, CaCl2 0.2 M and 14-16% PEG1000, VAPOR DIFFUSION, SITTING DROP, temperature 293K