4RGA
Phage 1358 receptor binding protein in complex with the trisaccharide GlcNAc-Galf-GlcOMe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-10 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 166.370, 166.370, 166.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.000 - 2.100 |
| R-factor | 0.1811 |
| Rwork | 0.180 |
| R-free | 0.19180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l92 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.110 | 0.750 |
| Number of reflections | 89232 | |
| <I/σ(I)> | 12 | 2.1 |
| Completeness [%] | 99.9 | 98.5 |
| Redundancy | 11.4 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 13 mg/ml in a solution containing 10 mM Zinc Sulfate Heptahydrate, 100 mM MES buffer pH 6.5., VAPOR DIFFUSION, SITTING DROP, temperature 293K |
