4RFX

Crystal Structure of the Dynactin DCTN1 Fragment involved in Dynein Interaction

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	155.320, 155.320, 129.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.700 - 2.900
R-factor	0.25935
Rwork	0.258
R-free	0.27705
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.623
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELXD
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.700	2.950
High resolution limit [Å]	2.900	2.900
Number of reflections	35585
Completeness [%]	99.9	99.88
Redundancy	26.3	27.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1 M Na-citrate pH 5.5, 5% PEG 4000, 0.1 M ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1 M Na-citrate pH 5.5, 5% PEG 4000, 0.1 M ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 293K