4RFU

Crystal structure of truncated P-domain from Grouper nervous necrosis virus capsid protein at 1.2A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A
Synchrotron site	NSRRC
Beamline	BL15A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-08
Detector	RAYONIX MX300HE
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	64.982, 83.458, 85.577
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.762 - 1.200
R-factor	0.1753
Rwork	0.175
R-free	0.18070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wiz
RMSD bond length	0.008
RMSD bond angle	1.044
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.210
High resolution limit [Å]	1.200	1.200
Number of reflections	144895
Completeness [%]	99.4	91

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.2M Ca acetate, 0.1M MES, pH 6.5, 10% (w/v) PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 291K