4RFS
Structure of a pantothenate energy coupling factor transporter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97913 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.726, 145.240, 157.141 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.500 - 3.232 |
R-factor | 0.2291 |
Rwork | 0.226 |
R-free | 0.28890 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.476 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.500 | 3.370 |
High resolution limit [Å] | 3.250 | 3.250 |
Rmerge | 0.080 | 0.884 |
Number of reflections | 28312 | |
<I/σ(I)> | 21.4 | 1.9 |
Completeness [%] | 97.6 | 97.6 |
Redundancy | 4.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.4 | 293 | 0.1 M Tris, 20% PEG2000, 15% glycerol, 0.2M MgCl2, pH 8.4, VAPOR DIFFUSION, temperature 293K |