4RFS
Structure of a pantothenate energy coupling factor transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97913 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.726, 145.240, 157.141 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.500 - 3.232 |
| R-factor | 0.2291 |
| Rwork | 0.226 |
| R-free | 0.28890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.476 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.500 | 3.370 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.080 | 0.884 |
| Number of reflections | 28312 | |
| <I/σ(I)> | 21.4 | 1.9 |
| Completeness [%] | 97.6 | 97.6 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.4 | 293 | 0.1 M Tris, 20% PEG2000, 15% glycerol, 0.2M MgCl2, pH 8.4, VAPOR DIFFUSION, temperature 293K |
