4RF9
Crystal structure of double-domain arginine kinase from Anthopleura japonicas in complex with L-arginine and ATPgS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.263, 59.483, 162.148 |
Unit cell angles | 90.00, 91.36, 90.00 |
Refinement procedure
Resolution | 47.955 - 2.350 |
R-factor | 0.2017 |
Rwork | 0.199 |
R-free | 0.24770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rf6 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.702 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 2.390 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 60449 | |
Completeness [%] | 96.9 | 98.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 20mM HEPES, 50mM NaCl, 10mM L-arginine, 10mM ATPgS, 0.2M Potassium Acetate, 20% PEG3350, pH 7.8, VAPOR DIFFUSION, HANGING DROP |