4RF7

Crystal structure of double-domain arginine kinase from Anthopleura japonicas in complex with substrate L-arginine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-04-12
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54178
Spacegroup name	P 1 21 1
Unit cell lengths	78.500, 59.271, 162.256
Unit cell angles	90.00, 91.31, 90.00

Refinement procedure

Resolution	47.855 - 2.100
R-factor	0.1821
Rwork	0.180
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rf6
RMSD bond length	0.004
RMSD bond angle	0.802
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.900	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	87800
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8		20mM Tris, 50mM NaCl, 10mM, L-arginine, 200mM Potassium Acetate, 20% PEG3350, pH 7.8, VAPOR DIFFUSION, HANGING DROP