4REY
Crystal Structure of the GRASP65-GM130 C-terminal peptide complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2013-12-09 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 61 |
Unit cell lengths | 129.725, 129.725, 37.104 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 64.860 - 1.960 |
R-factor | 0.155 |
Rwork | 0.152 |
R-free | 0.19000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4kfv |
RMSD bond length | 0.018 |
RMSD bond angle | 1.774 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.840 | 2.030 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.103 | 0.481 |
Number of reflections | 25936 | |
<I/σ(I)> | 13.78 | 4.39 |
Completeness [%] | 99.6 | 100 |
Redundancy | 13.1 | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 291 | 30% PEG 4000, 0.1M Tris, 0.2M Lithium sulfate, , pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |