4REO

Mutant ribosomal protein l1 from thermus thermophilus with threonine 217 replaced by valine

Replaces:  3U42

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-16
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 21 21 2
Unit cell lengths	75.990, 60.450, 43.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.855 - 1.350
R-factor	0.1694
Rwork	0.167
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ad2
RMSD bond length	0.006
RMSD bond angle	1.116
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1690))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.670	1.420
High resolution limit [Å]	1.350	1.350
Rmerge	0.025	0.388
Number of reflections	44873
<I/σ(I)>	25.16	3.53
Completeness [%]	99.6	100
Redundancy	3.96	3.95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10	295	15 MG/ML L1 50 MM GLYCINE, INITIALLY AT PH 10 5% (V/V) METHANE PENTANEDIOL 1.2 M AMMONIUM SULFATE EQUILIBRATED AGAINST 2.4 M AMMONIUM SULFATE 7% (V/V) METHANE PENTANEDIOL, VAPOR DIFFUSION, HANGING DROP, temperature 295K