4RDX
Structure of histidinyl-tRNA synthetase in complex with tRNA(His)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 84.061, 159.703, 123.988 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.469 - 2.550 |
R-factor | 0.21 |
Rwork | 0.207 |
R-free | 0.26170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1H4V and 3WC1 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.800 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.550 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.148 | 0.700 |
Number of reflections | 27099 | |
<I/σ(I)> | 13.9 | 2.6 |
Completeness [%] | 98.8 | 98.8 |
Redundancy | 5.4 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4.5 | 298 | 5% PEG4000, 0.1M NaOAc pH4.5, EVAPORATION, temperature 298K |