4RDV

The structure of N-formimino-L-Glutamate Iminohydrolase from Pseudomonas aeruginosa complexed with N-formimino-L-Aspartate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-03
Detector	ADSC QUANTUM 4
Wavelength(s)	1.28192
Spacegroup name	C 1 2 1
Unit cell lengths	304.047, 67.248, 98.231
Unit cell angles	90.00, 91.50, 90.00

Refinement procedure

Resolution	45.509 - 2.080
R-factor	0.1456
Rwork	0.144
R-free	0.18310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.038
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.509
High resolution limit [Å]	2.080
Number of reflections	119180
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	293	1.4M SODIUM PHOSPHATE, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K