4RDR
Structure of the bacterial Zn-transporter ZnuD from Neisseria meningitidis (locked conformation bound to zinc and cadmium ions)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-21 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 101.073, 155.819, 159.607 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.981 - 2.472 |
| R-factor | 0.2193 |
| Rwork | 0.218 |
| R-free | 0.24780 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.172 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.530 |
| High resolution limit [Å] | 2.470 | 6.090 | 2.470 |
| Rmerge | 0.113 | 0.065 | 0.963 |
| Number of reflections | 45376 | ||
| <I/σ(I)> | 12.1 | ||
| Completeness [%] | 100.0 | 99.6 | 100 |
| Redundancy | 12.2 | 12.3 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 300 | 0.1M sodium cacodylate pH 6.6, 0.4M Magnesium Sulfate, 2% (w/v) Ethylene glycol, 0.7mM Cadmium chloride, vapor diffusion, hanging drop, temperature 300K |
