4RDP
Crystal structure of Cmr4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 41 |
| Unit cell lengths | 63.035, 63.035, 189.073 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.569 - 2.850 |
| R-factor | 0.1852 |
| Rwork | 0.180 |
| R-free | 0.23490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.544 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.570 | 2.952 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Number of reflections | 15822 | |
| Completeness [%] | 92.2 | 59.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 303 | 10% PEG8000, 6% MPD, 0.1M Tris pH8.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
