4RDO

Structure of YTH-YTHDF2 in the free state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 65
Unit cell lengths	139.280, 139.280, 112.950
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.280 - 2.150
R-factor	0.2156
Rwork	0.213
R-free	0.27020
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.256
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	SOLVE
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	120.620	42.276	2.270
High resolution limit [Å]	2.150	6.800	2.150
Rmerge		0.037	0.542
Total number of observations		9502	43694
Number of reflections	65587
<I/σ(I)>	10.3	13.2	1.4
Completeness [%]	97.0	91.8	98.4
Redundancy	4.5	4.7	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	283	0.2 M Potassium tartrate, 0.1 M Sodium citrate tribasic dehydrate, PH 5.6, 2M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 283K