4RD9

X-RAY STRUCTURE OF THE APO FORM OF THE AMYLOID PRECURSOR PROTEIN-LIKE PROTEIN 1 (APLP1) E2 DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-08
Detector	RAYONIX MX-225
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	66.425, 79.145, 95.201
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.997 - 2.600
R-factor	0.2169
Rwork	0.215
R-free	0.24730
RMSD bond length	0.003
RMSD bond angle	0.637
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.760	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.074	0.367
<I/σ(I)>	17.8	5.3
Completeness [%]	100.0	100
Redundancy	7.3	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293.15	0.1 M Na-Citrate, 0.9 M(NH4)H2PO4, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K