4RD3

Structure of aIF2-gamma H97A variant from Sulfolobus solfataricus bound to GDP and Pi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-04
Detector	PSI PILATUS 6M
Wavelength(s)	0.979180
Spacegroup name	P 21 21 21
Unit cell lengths	46.320, 60.460, 144.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.363 - 1.690
R-factor	0.1637
Rwork	0.161
R-free	0.22280
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4rd1
RMSD bond length	0.007
RMSD bond angle	1.074
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.8.4_1496)
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.360	1.780
High resolution limit [Å]	1.680	1.680
Rmerge	0.070	0.560
Number of reflections	46188
<I/σ(I)>	12.85	1.74
Completeness [%]	97.3	91.9
Redundancy	3.44	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	297	60%MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K