4RCW
Crystal structure of human Slitrk1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.99362 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 69.607, 69.607, 409.946 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.567 - 3.192 |
| R-factor | 0.2605 |
| Rwork | 0.259 |
| R-free | 0.29750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m18 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.260 |
| High resolution limit [Å] | 3.192 | 8.670 | 3.192 |
| Number of reflections | 10569 | ||
| <I/σ(I)> | 35 | ||
| Completeness [%] | 99.0 | 98.5 | 100 |
| Redundancy | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 296 | 100 mM Na-citrate, pH 5.5, 16% isopropanol, and 12% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 296.0K |
