4RCM
Crystal structure of the Pho92 YTH domain in complex with m6A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-04-15 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.430, 57.171, 66.725 |
| Unit cell angles | 90.00, 103.08, 90.00 |
Refinement procedure
| Resolution | 32.970 - 1.800 |
| R-factor | 0.22 |
| Rwork | 0.218 |
| R-free | 0.26810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 4R3H AND 4RCI |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.595 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.17) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.400 | 38.400 | 1.840 |
| High resolution limit [Å] | 1.800 | 9.010 | 1.800 |
| Rmerge | 0.090 | 0.029 | 0.391 |
| Total number of observations | 791 | 4918 | |
| Number of reflections | 27985 | ||
| <I/σ(I)> | 10.9 | 35.3 | 4.1 |
| Completeness [%] | 99.5 | 97.5 | 92.9 |
| Redundancy | 3.6 | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG-3350, 0.2 M potassium isocyanate, vapor diffusion, sitting drop, temperature 293K |
