4RCA

Crystal structure of human PTPdelta and human Slitrk1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-11
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	76.981, 60.195, 83.679
Unit cell angles	90.00, 102.19, 90.00

Refinement procedure

Resolution	38.615 - 2.991
R-factor	0.2628
Rwork	0.263
R-free	0.26910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rcw 2yd6
RMSD bond length	0.016
RMSD bond angle	0.846
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.050
High resolution limit [Å]	2.991	8.130	2.991
Number of reflections	15130
<I/σ(I)>	14.1
Completeness [%]	98.6	96.5	95.9
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	296	100 mM Tris-Cl, pH 8.5, 200 mM ammonium sulfate, and 20% PEG3350 (v/v), VAPOR DIFFUSION, HANGING DROP, temperature 296.0K