4RBN
The crystal structure of Nitrosomonas europaea sucrose synthase: Insights into the evolutionary origin of sucrose metabolism in prokaryotes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2012-08-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.008 |
| Spacegroup name | P 65 |
| Unit cell lengths | 236.899, 236.899, 213.442 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 68.387 - 3.050 |
| R-factor | 0.1786 |
| Rwork | 0.176 |
| R-free | 0.22820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.537 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.883 | |
| High resolution limit [Å] | 3.050 | 3.050 |
| Number of reflections | 126170 | |
| Completeness [%] | 97.9 | 97.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 293 | 5% Tacsimate pH 5.0, 5% (w/v) PEG 3350, and 0.1 M sodium citrate pH 5.6, VAPOR DIFFUSION, temperature 293K |
