4RAE
Crystal structure of Rv1600 encoded aminotransferase from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ DW |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-12-08 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 159.937, 159.937, 110.317 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.520 - 2.590 |
| R-factor | 0.24077 |
| Rwork | 0.239 |
| R-free | 0.27870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cq5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.409 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 2.690 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Number of reflections | 47703 | |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 94.0 | 85.3 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.2M trimethylamine N-oxide dehydrate, 0.1M Tris pH 8.5, 30% PEG MME 2000, 0.02M EDTA, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
