4RAA
Crystal structure of a Putative exported protein (BF0058) from Bacteroides fragilis NCTC 9343 at 2.60 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91837,0.97947,0.97908 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.800, 55.210, 81.330 |
| Unit cell angles | 90.00, 122.27, 90.00 |
Refinement procedure
| Resolution | 61.243 - 2.600 |
| R-factor | 0.2162 |
| Rwork | 0.214 |
| R-free | 0.25710 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.713 |
| Data reduction software | XDS |
| Data scaling software | XSCALE (January 10, 2014 BUILT=20140307) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 61.243 | 2.670 | |
| High resolution limit [Å] | 2.600 | 11.640 | 2.600 |
| Rmerge | 0.073 | 0.014 | 0.649 |
| Number of reflections | 14464 | 285 | 1992 |
| <I/σ(I)> | 8.59 | 39.8 | 1 |
| Completeness [%] | 92.8 | 89.3 | 92.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.250M di-ammonium hydrogen phosphate, 22.1% polyethylene glycol 3350, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
